Formulation of medium : 96
MEDIUM 96 - for Mycobacterium avium subsp. paratuberculosis
-
Middlebrook 7H9 broth 4.7 g (
BECTON DICKINSON
271310)
-
Tween 80 0.5 ml (
Merck
822187)
-
Middelbrook OADC enrichment 100 ml (
Becton Dickinson
211886)
-
Mycobactine 2 ml (
ID VET
MYCO)
-
Distilled water 900 ml
Add all products except OADC and mycobactin Adjust pH to 6.6 + 0.2 Autoclave at 121°C for 15 minutes Cool to 45°C Add aseptically OADC Supplement and Mycobactin
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